Year: 2014 | Month: March and June | Volume 2 | Issue 1 and 2
Genetic Diversity Analysis and RAaPD Marker Inheritance in F1 Progenies of Mulberry (Morus spp.)
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Abstract:
The present study attempts to understand the pattern of RAPD markers inheritance in the F1 progenies of a cross Mysore Local and V-1 of mulberry (Morus spp.). RAPD marker analysis was carried out using 5 decamer random primers. Out of 33 amplicons generated, 20 were of monomorphic and rest 13 were polymorphic (39:39%). The size of the amplified products ranged from 500-3200 bp. The genetic distance (GD) between the parents based on RAPD data set is 0.186, which is low in comparison with morpho-agronomical divergence. In some progenies, GD calculated was higher than the parents suggesting OPA-022900, OPA-02900, OPA-02800 RAPD markers showed expected Mendelian pattern of inheritance. Besides three other markers, OPA-041450, OPA-041000 and OPA-071000 showed Mendelian segregation pattern for 1:1 ratio. The finding proves that the dominant status of the RAPD markers may not be a limiting factor in genetical analysis of a heterozygous tree like mulberry, where F1 tends to behave like a segregating population. The polymorphic RAPD markers in parents, segregates as independent alleles in the F1 progenies and showed the heterozygotic status of mulberry varieties.
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